{"id":6109,"date":"2026-11-06T13:31:35","date_gmt":"2026-11-06T12:31:35","guid":{"rendered":"https:\/\/www.woodresearch.sk\/cms\/?p=6109"},"modified":"2026-11-06T13:31:37","modified_gmt":"2026-11-06T12:31:37","slug":"analysis-of-structural-characteristics-of-bacterial-nanocellulose-synthesized-using-beechwood-hydrolysate","status":"publish","type":"post","link":"https:\/\/www.woodresearch.sk\/cms\/analysis-of-structural-characteristics-of-bacterial-nanocellulose-synthesized-using-beechwood-hydrolysate\/","title":{"rendered":"Analysis of Structural Characteristics of Bacterial Nanocellulose Synthesized Using Beechwood Hydrolysate"},"content":{"rendered":"\n<div class=\"wp-block-pdf-viewer-block-standard\" style=\"text-align:left\"><div class=\"uploaded-pdf\"><a href=\"https:\/\/www.woodresearch.sk\/cms\/wp-content\/uploads\/2026\/11\/1_Analysis-of-Structural-Characteristics-of-Bacterial-Nanocellulose-Synthesized-Using-Beechwood-Hydrolysate.pdf\" data-width=\"\" data-height=\"\"><\/a><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>In this study, a comprehensive structural analysis to increase yield, optimize cultivation parameters, and evaluate bacterial nanocellulose (BNC) organization using two types of media was conducted. Cultivation of Komagataeibacter xylinus (K. xylinus) DSM 46591 was performed in an alternative beechwood hydrolysate medium (BH) and the standard Hestrin\u2013Schramm medium (HS) over a period of 72\u2013432 h. High\u2013performance liquid chromatography (HPLC) confirmed the successful extraction of glucose, xylose, and associated compounds from the wood. Fourier\u2013transform infrared spectroscopy (FTIR) revealed visible changes in supramolecular structure in BH, resulting in a 12.8% decrease in the lateral order index (LOI: 0.748 \u2192 0.652) and a simultaneous increase in the hydrogen bond index (HBI: 0.997 \u2192 1.111) in comparison with HS. X\u2013ray diffraction (XRD) showed that BH promotes the synthesis of a\u00a0combination of cellulose triclinic (I\u03b1) and monoclinic (I\u03b2) with a high crystallinity index (CI\u00a0= 86%), while sterically limiting lateral crystallite growth (the crystallite sizes (CS) of the (002) crystallographic plane were 6.1\u20136.5 nm for BH vs. 6.7 nm for HS). Scanning electron microscopy (SEM) confirmed that, in BH, this resulted in a reticulated, interconnected three\u2013dimensional nanofibrillar matrix with preserved capillary porosity. In contrast, the HS samples showed pore clogging, denser fibre packing, and layer stacking.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5713],"tags":[5720,5718,5719,5721,5722],"release_year":[5476],"ppma_author":[5714,5715,418,5716,4803,192,4802,344,5717],"class_list":["post-6109","post","type-post","status-publish","format-standard","hentry","category-wood-research-volume-71-number-4-2026","tag-bacterial-nanocellulose-bnc","tag-beechwood-hydrolysate-bh","tag-enzymatic-hydrolysis-eh","tag-hestrin-schramm-medium-hs","tag-komagataeibacter-xylinus-k-xylinus-dsm-46591","release_year-5476"],"featured_image_src":null,"author_info":{"display_name":"Ivanke","author_link":"https:\/\/www.woodresearch.sk\/cms\/author\/ivanke\/"},"authors":[{"term_id":5714,"user_id":0,"is_guest":1,"slug":"eduard-horban","display_name":"Eduard Horban","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":5715,"user_id":0,"is_guest":1,"slug":"petra-olejnikova","display_name":"Petra Olejn\u00edkov\u00e1","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":418,"user_id":0,"is_guest":1,"slug":"albert-russ","display_name":"ALBERT RUSS","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":5716,"user_id":0,"is_guest":1,"slug":"michal-zitnan","display_name":"Michal \u017dit\u0148an","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":4803,"user_id":0,"is_guest":1,"slug":"stefan-bohacek","display_name":"\u0160TEFAN BOH\u00c1\u010cEK","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":192,"user_id":0,"is_guest":1,"slug":"vladimir-ihnat","display_name":"VLADIM\u00cdR IHN\u00c1T","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":4802,"user_id":0,"is_guest":1,"slug":"andrej-pazitny","display_name":"ANDREJ PA\u017dITN\u00dd","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":344,"user_id":0,"is_guest":1,"slug":"monika-stankovska","display_name":"MONIKA STANKOVSK\u00c1","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":5717,"user_id":0,"is_guest":1,"slug":"jan-talaba","display_name":"J\u00e1n Tal\u00e1ba","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/posts\/6109","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/comments?post=6109"}],"version-history":[{"count":1,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/posts\/6109\/revisions"}],"predecessor-version":[{"id":6111,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/posts\/6109\/revisions\/6111"}],"wp:attachment":[{"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/media?parent=6109"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/categories?post=6109"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/tags?post=6109"},{"taxonomy":"release_year","embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/release_year?post=6109"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/www.woodresearch.sk\/cms\/wp-json\/wp\/v2\/ppma_author?post=6109"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}