COMPARISON OF TWO METHOD FOR ISOLATION OF FIBRILLATED CELLULOSE FROM LIGNOCELLULOSIC BIOMASS

Fibrillated cellulose from distillery refuse based on maize starch was prepared by two different procedures. The effect of sonification was evaluated atacid-alkali extractionas well as the type of used acid. The results from the alkali-acid procedure were compared with these obtained by method of steam explosion at different temperatures. The acid-alkali method brings a better result regarding degradation of hemicellulose and lignin as well as cellulose. Lignin/hemicellulose were only released from lignocellulose network using steam explosion at 120-180°C. At higher temperature, the results were comparable with those obtained by acid-alkali method. Similarly pore size distribution of filter paper decreased more significantly when fibrillated cellulose from acid-alkali treatment was applied. After steam explosion, higher extend of longer still fibres remains

ENZYMATIC HYDROLYSIS OF STEAM EXPLODED STRAW WITH THE ADDITION OF ACETIC ACID

The effect of steam explosion on the enzymatic hydrolysis of straw was investigated in the presence of 5, 10, 15 and 20% wt. addition of acetic acid. Analysis was performed at temperatures of 160, 170, 180, 190, 200 and 210°C. The concentration of monosaccharides obtained after enzymatic hydrolysis was considered the main indicator of the increased availability of cellulose due to their release into the solution. The results indicate that the addition of acetic acid increases the concentration of monosaccharides, but only at lower temperatures. The temperature of 180°C corresponded to the most effective pretreatment by steam explosion in the presence of acetic acid with the highest concentration of 10%, which corresponds to the conversion of polysaccharides to monosaccharides of 74.78%. At high temperatures above 200°C, the addition of acetic acid results in a decrease in the concentration of monosaccharides due to the high severity factor in the range of 3.94 – 4.24