Wood degrading mushrooms potentially strong towards laccase biosynthesis in Pakistan
In present study, Pleurotus ostreatus, Ganoderma lucidum, Ganoderma ahmadii, Ganoderma applanatum, Ganoderma australe, Ganoderma colossus, Ganoderma flexipes, Ganoderma resinaceum, Ganoderma tornatum, Trametes hirsutus, Trametes proteus, Trametes pubescens, Trametes tephroleucus, Trametes versicolor, Trametes insularis, Fomes fomentarius, Fomes scruposus, Fomitopsis semitostus, Fomes lividus, Fomes linteus, Phellinus allardii, Phellinus badius, Phellinus callimorphus, Phellinus caryophylli, Phellinus pini, Phellinus torulosus, Poria ravenalae, Poria versipora, Poria paradoxa, Poria latemarginata, Heterobasidion insulare, Schizophyllum commune, Schizophyllum radiatum, Daldinia sp., Xylaria sp., were collected, isolated, identified and then screened qualitatively for their laccase activity. Among all the collected and tested fungi Pleurotus ostreatus 008 and 016, Ganoderma lucidum 101,102 and 104 were highly efficient in terms of laccase production. The potent strains were further subjected to Quantitative laccase bioassay for partial purification and characterization of industrially important enzyme.