2D-DIGE identification of proteins in wood-forming tissue of Pinus koraiensis seedling stem after artificial bending treatment
Compression wood is an ideal model for exploring the molecular mechanism of wood formation. To supplement the proteome data in compression wood formation, two-dimensional difference gel electrophoresis (2D-DIGE) proteome technology was used to investigate the protein response to bending the stem of Pinus koraiensis seedlings. The results showed that most of the proteins in wood-formation tissues were distributed in pH from 4-8 and the molecular weight was around 30-97 KDa. In total 24 identified proteins were mainly functional on amino acid metabolism, cell wall synthesis, secondary metabolism, and stress response. Proteins related to methionine pathway and lignin biosynthesis were up-regulated in the formation of the compression wood. On the contrary, lipid metabolism-related proteins were down-regulated during the formation of the compression wood. In additional, some proteins involved in energy metabolism and photosystem were also changed in the tissues during the formation of the compression wood. These findings suggested that 2D-DIGE was a feasible and timesaving technology in proteome analysis of wood-forming tissue. Moreover, proteins were not involved in lignin synthesis pathways, but other metabolites were changed as a response to stem bent treatment. The identified proteins in compression wood formation insight to further investigating the molecular mechanism of wood cell wall biosynthesis.